细菌发酵L乳酸的研究(3)

发布时间:2021-06-06

细菌发酵L乳酸的研究

自{『菌发酵L(+)一乳酸的研究

Abstract

A。bstract

Afterscreening,astereoisometricallypureL(+)一lacticacidproducingstrainS3wasisolated.Itwaspreliminaryidentified

as

Lactobacillussp.Aftermultiplemutagenesis

on

(UV.,NTG,LiCIandDES)andscreeningwiththemethodbased

analysis

itspathway

whose

andmetabolicregulation,strainM7

was

successfullyobtained

productivityofL(+)-lacticacidWasimprovedby30%.Auxotrophdeterminationshowedthatitwasaminoaciddeficient.TheoptimumconditionfermentNionrTwo—level

was

andmediaforM7

design

investigated

withthe

application

ofPlacea—Burman

design)andBox—Behnkendesign(ResponseSurfaceAnalysis)wi也the

helpofSAS(Statistical

Analysis

System)software,theyieldincreasedby

15%.The

kineticmodelofbatchfermentationWasprimarilyestablished.Undertheoptimumlevel

determined

bythismethod,afterfermentationfor72hours,aproductivityof

92-100g/LL(+)一lacticacid

Can

be

achieved.Theanion—exchange

resinD301Gwas

improvetheIn

selectedtoseparatethelacticacidfrom

Situ

fermentationbroth.Inorderto

ProductRemovaltechniquewith

to

ionexchangeresinwhichalleviatedthe

inhibition

bacteriagrowth

as

wellasproductformation,extractive

fermentation

usingimmobilizedcellinCa2+.alginategelWasalsostudied.

Keywords:lacticacidbacteria,L(+) lacticacid,multiplemutagenesis;mutation,

Placer burmandesign,ResponseSurfaceAnalysis,immobilizedcell,InSituProductRemoval

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