目 录

缩略语表..................................................................................................................................1

中文摘要..................................................................................................................................3

Abstract.....................................................................................................................................6

Introduction...............................................................................................................................9

文献回顾................................................................................................................................11

1 转录组学概述............................................................................................................11

2 转录组学研究的传统方法.........................................................................................12

3 基于新一代测序技术的高通量转录组学研究方法.................................................29

4 肝癌的转录组学研究.................................................................................................40

Study contents.........................................................................................................................42

Part 1：HCC sample preparation and RNA-sequencing....................................................42

1 materials.......................................................................................................................42

1.1 Patients and tissue specimens...............................................................................42

1.2 Regents..................................................................................................................42

1.3 Experimental equipment.......................................................................................43

2 Methods.......................................................................................................................43

3 Results..........................................................................................................................52

3.1 The clinical features of the patients......................................................................52

3.2 Total RNA concentration and integrity test...........................................................53

3.3 RNA-seq analysis of 10 matched pairs of HCC and adjacent non-cancerous tissues....54

3.4 evaluation of data quality and sequencing depth required for transcriptome analysis...55

3.5 Comparison between RNA-seq and microarray....................................................57

4 Discussion....................................................................................................................59

Part 2: Global analysis of HCC transcriptome.................................................................60

1 Material........................................................................................................................60

1.1 Experimental Equipment.......................................................................................60

1.2 Reagents................................................................................................................60

2 Method.........................................................................................................................60