Transcriptome analysis of HCC by

next-generation sequencing

(RNA-seq analyses generate comprehensive transcriptomic

landscape and reveal complex transcript patterns in

hepatocellular carcinoma)

Doctorial Cancidate: Hanqiang Liu

Supervisor: Prof. /Academician Zhinan Chen

Assistant tutor: Jinliang Xing

Departmen of Cell Biology & Cell Engineering Research Center, Fourth

Military Medical University, Xi,an, 710032, China

Abstract

RNA-seq is a powerful tool for comprehensive characterization of whole

transcriptome at both gene and exon levels and with a unique ability of

identifying novel splicing variants. To date, RNA-seq analysis of HBV-related

hepatocellular carcinoma (HCC) has not been reported. In this study, we

performed transcriptome analyses for 10 matched pairs of cancer and

non-cancerous tissues from Chinese patients with HBV-related HCC using 36bp

single-end sequencing approach on Solexa/Illumina GAII platform. On average,