华　中　科　技　大　学　博　士　学　位　论　文

Part I

Expression and significance of carbonic anhydrases IX and vascular endothelial growth factor in non-small cell lung cancer

【Abstract】Objective: To explore the expression and role of carbonic anhydrases IX (CAIX) and vascular endothelial growth factor (VEGF) in non-small cell lung cancer. Methods: The expression of CAIX and VEGF in 86 pathologically diagnosed non-small cell lung can cer and normal lung tissue was detected using immunohistochemical technique. The immunostaining data was compared with clinical pathological parameters. Results: Among the 84 specimen of malignant cases, expression of CAIX was detected in 60(71.4%) and VEGF in 56(66.7%). Both CAIX and VEGF were expressed maximally on the luminal surface of tumors and around regions of necrosis in tumors. No positive staining of CAIX and VEGF were found in normal lung tissue. The expressions of CAIX and VEGF were related to tumor size, clinical stage and status of lymphatic node. There was an association between CAIX and VEGF(r=0.405,P<0.01). Conclusion: The hypoxia and angiogenesis are important markers of carcinoma. CAIX and VEGF were involved in the tumorgenesis and progression of non-small cell lung cancer. CAIX and VEGF might play synergetic roles in invasion and metastasis of the disease.

【Key Words】non-small cell lung cancer; carbonic anhydrase; vascular endothelial growth factor; immunohistochemistry

Part II

The detection of vascular endothelial growth factor and endostatin in malignant and tuberculosis pleural effusions

【Abstract】Objective: To explore changes and clinical significance of VEGF and endostatin in malignant and tuberculosis pleural effusions. Methods: Eighty-two effusion samples, 42 from patients with lung cancer and 40 from patients with tuberculosis, were analyzed. VEGF and endostatin concentrations were determined by enzyme linked immunoabsent assay (ELISA) or competitive enzyme immunoassay. And sensitivity, specificity and accuracy for diagnosing the malignant pleural effusion were compared.

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