亚粒径阳离子交换色谱(9)

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9 MAb separation on Proteomix ® SCX NP1.7 4.6 x 100 mm vs. NP5 4.6 x 250 mm

Figure 12. Particle size and column length comparison for Proteomix ® SCX NP. Mobile phase A: 2.4 mM Tris, 1.5 mM Imidazole, 11.6 mM piperazine pH 6.0 and B: A + 0.5 M NaCl pH 10.5. The gradient was 0-100% B in 25 minutes with a 15 minute prewash. Flow rate was 0.75 mL/min (NP1.7) and 0.8 mL/min (NP5). UV detection was set at 280 nm. 5 µL of MAb 321 was injected on each column (1 mg/mL).

MAb separation on Proteomix ® SCX NP1.7 vs. Proteomix ® SCX NP5 (4.6 x 100 mm)

Figure 13. Particle size comparison for Proteomix ® SCX NP1.7 4.6 x 100 mm to Proteomix ® SCX NP5 4.6 x 100 mm. Mobile phase A: 2.4 mM Tris, 1.5 mM Imidazole, 11.6 mM piperazine pH 6.0 and B: A + 0.5 M NaCl pH 10.5. The gradient was 0-100%

B in 25 minutes with a 15 minute prewash. Flow rate was 0.75 mL/min. UV detection was set at 280 nm. 5 µL of MAb 321 was injected on each column (5

mg/mL).