亚粒径阳离子交换色谱(11)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

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11 Gradient Optimization for Peptide Separation on Proteomix ® SCX NP1.7

Figure 16. Gradient optimization for the separation of four Peptides on Proteomix ® SCX NP1.7 2.1 x 30 mm. Mobile phase A: 10 mM phosphate buffer pH 2.5, B: A + 1 M NaCl and C: 100% acetonitrile. Flow rate was 0.35 mL/min. UV detection was set at 214 nm. 2 µL of the peptide mixture was injected.

Particle Size Comparison for the Separation on Peptides on Proteomix ® SCX

Figure 17. Particle size comparison for Proteomix ® SCX NP1.7 2.1 x 30 mm to Proteomix ® SCX NP5 4.6 x 100 mm. Mobile phase A: 10 mM phosphate buffer pH 2.5, B: A + 1 M NaCl and C: 100% acetonitrile. Flow rate was 1.0 mL/min (for 5 µm) and 0.35 mL/min (for 1.7 µm). UV detection was set at 214 nm. 2 µL of the peptide mixture was injected.

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