亚粒径阳离子交换色谱(8)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

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8 Column Lifetime for Proteomix ® SCX 4.6 x 50 mm

Figure 10. Lifetime test for Proteomix ® SCX NP1.7 4.6 x 50 mm with a 4 x 10 mm guard. Mobile phase A: 20 mM sodium phosphate buffer pH 6.5 and B: A + 1.0 M NaCl. The gradient was 0-100% B in 25 minutes with a 15 minute prewash. Flow rate was 0.5 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL each).

Column Lifetime for Antibodix ™ WCX 4.6 x 50 mm

Figure 11. Lifetime test for Antibodix ™ WCX NP1.7 4.6 x 50 mm with a 4 x 10 mm guard column. Mobile phase A: 20 mM sodium phosphate buffer pH 6.5 and B: A + 1.0 M NaCl. The gradient was 10-100% B in 25 minutes with a 15 minute prewash. Flow rate was 0.3 mL/min. UV detection was set at 214 nm. 3 µL of sample was injected and the sample is a mixture of Aprotinin, Lysozyme and Ribonuclease A (1 mg/mL

each).