亚粒径阳离子交换色谱(15)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

15 Particle Size Comparison for the Separation of MAb on Antibodix ™ WCX

Figure 23. Comparison of Antibodix ™

WCX NP5 4.6 x 100 mm (without a guard) to Antibodix WCX NP1.7 4.6 x 100 mm (with a 4 x 10 mm guard). Mobile phase A:

20 mM Sodium Acetate pH 5.15 and B: A + 1 M LiCl. Flow rate was 0.4 mL/min for both columns. UV detection was set at 280 nm. 25 µg of MAb 321 was injected on each column for analysis. Gradient Optimization for MAb Separation on Antibodix ™ WCX NP1.7

Figure 24. Gradient optimization for the analysis of MAb on Antibodix ™

WCX NP1.7

4.6 x 100 mm. Mobile phase A: 20 mM Sodium phosphate pH 7.5 and B: A + 1 M NaCl. Flow rate was 0.4 mL/min. UV detection was set at 280 nm. 2 µL of MAb 016 was injected for each run (11 mg/mL).