亚粒径阳离子交换色谱(14)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

Particle Size Comparison for the Separation of MAb on Antibodix™ WCX Figure 21. Comparison of Antibodix™WCX NP5 4.6 x 250 mm (without a guard) to Antibodix WCX NP1.7 4.6 x 100 mm (with a 4 x 10 mm guard). Mobile phase A:

20 mM Sodium Acetate pH 5.15 and B: A + 1 M LiCl. Flow rate was 0.4 mL/min (NP1.7) and 0.8 mL/min (NP5). UV detection was set at 280 nm. MAb 321 was injected on each column for analysis.

MAb Loading Study on Antibodix™ WCX NP1.7 4.6 x 100 mm

Figure 22. MAb Loading Study on Antibodix™WCX NP1.7 4.6 x 100 mm. Mobile phase A: 20 mM Sodium Acetate pH 5.15 and B: A + 1 M LiCl. Flow rate was

0.4 mL/min. UV detection was set at 280 nm. 5 µL, 2 µL and 1 µL of MAb 321 (5 mg/mL) were injected for analysis.

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