亚粒径阳离子交换色谱(13)

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13 Analysis of Fab and Fc Fragments on Proteomix ® WCX NP1.7

Figure 20. Analysis of papain digested MAb fragments, Fab and Fc, on Proteomix ® WCX NP1.7 4.6 x 30 mm. Mobile phase A: 20 mM Sodium Acetate pH 5.15, B: A + 1 M LiCl. Flow rate was 1.5 mL/min. UV detection was set at 280 nm. 25 µL of the papain digested MAb 321 (1 mg/mL) was injected. Digestion condition: 5.0 mM

L-Cysteine, 2.0 mM EDTA and 0.1M Tris-HCl pH 7.6 (Papain: MAb=100:1). Incubate at 37 °

C for 3.5 hours, add 5% TFA to stop the reaction and chill on ice for 15 minutes.