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[12] 刘蓉.双歧杆菌糖苷酶的研究[J].中国微生态学杂志.1996,8(6):42 -47. The Effecting of Chinese Traditional Medicine and Optimization of Culture Medium onBifidobacteriumMultiplication

ZHANG Huo-yun1, SUN Qi-ling1, YUAN Yue-xiang2,LIUXiao-feng2, CHEN Fei2

(1. College of Life Science, Sichuan University,Chengdu 610064,China;2 .Chengdu Inst. Biol. Ch-i

nese Academy of Sciences, Chengdu 610041,China)

Abstract:Spectrophotometric method was used to determine the growth promoting effect ofLycium bar-

barumandChinese yam.s extracts onBifidobacterium adolescentis. Two optimum reproducible culture

medium was optimized by quadratic design. The adding concentration of 50%Lycium barbarum.s extract

was 2%, and 25%Chinese yam3% . Under optimum culture condition , growth curve for strain was mea-

sured. By Hungate anaerobic cultivition and a concrete roll tube method detecting CFU in stationary phase,

CFU got to 2.21 108cfu/mL.

Key words:Bifidobacterium adolescentis;Chinese traditional medicine; multiplication;optimization of culture

medium

192四川大学学报(自然科学版)第41卷