人参皂甙 Rh2对人乳腺癌MCF7AdrR细胞增殖和凋亡的

人参皂甙 Rh2对人乳腺癌MCF7AdrR细胞增殖和凋亡的影响

人参皂甙- Rh2对人乳腺癌MCF7/AdrR细胞增殖和凋亡的影响

Efficacy ofGensenoside Rh2 on proliferation and apoptosis forMCF7 /AdrR human

breast cell line

摘要: 目的 研究人参皂甙- Rh2 ( GS- Rh2 ) 对乳腺癌MCF7 /AdrR细胞增殖和

凋亡的影响。方法 以MCF7 /AdrR细胞不加药物干预, 作为对照组; 以Rh2单独 作用于MCF7 /AdrR 细胞, 作为Rh2 组; 以低浓度阿霉素( ADM ) 单独作用于

MCF7 /AdrR细胞, 作为ADM 组; 以Rh2和阿霉素联用于MCF7 /AdrR 细胞, 作为 Rh2 + ADM 组。用MTT比色法观察GS- Rh2对体外培养的MCF7 /AdrR 细胞

生长的抑制作用; 用荧光显微镜观察用药前后MCF7 /AdrR 细胞的形态学变化;

用流式细胞仪检测凋亡细胞和细胞周期变化; 用W estern blot的方法检测Fas、

Bax、Bc l- 2及Bcl- xL蛋白表达的变化。结果 MCF7 /AdrR 细胞经GS- Rh2

作用后, 生长受抑制, 呈剂量和时间依赖性; 显微镜显示, 经GS- Rh2作用后,

MCF7 /AdrR细胞呈较明显的凋亡形态学改变, GS - Rh2能将细胞周期阻滞于

G0 /G1期, 同时可能参与促凋亡蛋白Fas、Bax 表达升高及抑制凋亡蛋白Bcl- 2

表达降低; 但在GS- Rh2处理前后Bc l- xL无明显变化。结论 GS - Rh2能抑

制体外培养的MCF7 /AdrR 细胞增殖并诱导其凋亡, 且这种作用可能是通过上调 Fas、Bax表达、下调B cl- 2表达以及阻滞细胞周期而实现。

关键词: 人参皂甙- Rh2; 乳腺癌; 细胞凋亡

中图分类号: R737 9; R979 1 文献标识码: A

文章编号: 1001- 6821( 2010) 12- 0923- 05

Abstract: Objective To exam ine the effect of GensenosideRh2 ( GSRh2

) on proliferation and apoptosisMCF7 /AdrR human breast cell line

Methods MCF7 /AdrR ce lls w ere d iv ided into contro l group, added w ith

normal saline; ADM group, added w ith adriamyc in(ADM ) at the low con

centration; Rh2 group, added w ith Rh2; and ADM + Rh2 group, added

w ith ADM at the low concen trat ion and Rh2 Ce ll grow th inh ib ition w as

evaluated by MTT co lorimetric assay C ellu larmorpho logy w as observed

underm icroscope Apoptot ic cells and the changes of cell cyclew ere de

term ined by flow cytom etry Western b lo tw as used to detect the prote in expression o f Fas, Bax, B cl- 2 and B cl- xL Results The inh ib itiono fGS - Rh2 on the grow th o fMCF7 /AdrR ce lls w as in a t ime- and

dose- dependent manner byMTT GS- Rh2 w as capab le o f arresting the cell cyc le at G0 /G1 phase Pro- apopto tic factors, Fas and Bax w ere re

markably h igher than contro l cells, wh ile the an ti- apopto tic factors, Bc l- 2 w ere sign ificantly low er than contro l ce lls, but the expression o f Bc l- xL prote in did no t change significan tly Conclusion GS- Rh2 exh ibited grow th inh ib ition e ffect and induced apoptosis onMCF7 /AdrR cell

line in vitroApoptosis ofMCF7 /AdrR cells induced by GS- Rh2 maybe

related w ith arrest ing ce ll cycle, up- regulating Fas, Bax protein expression and down- regulating mutated B cl- 2 pro

tein expression

Keywords: G ensenoside Rh2; breast cancer; apoptosis cell

近年研究发现[ 1, 2] , 人参具有抗癌活性, 其主要活性成分人参皂苷能诱导细胞分化和凋亡, 从而发挥抗