信号通路相关文献挖掘与分析方法研究(4)
发布时间:2021-06-05
发布时间:2021-06-05
(recallrate93%andprecisionrate27%)witll-2%,theprecisionrateishighwith-18%enhanced.We
2.Proteinfoundabout63.000interestedarticleswiththismethod.importantroleintransforminginformationofkinases(PKs)play
signalpathway.Theyphosphorylatethesubstrates(proteins)atthespecificsites(phosphorylationsites)flankingwithcanonicalmotif.Onceagain,Weminedliterature
USedescribingphosphorylatesitesbyusingtheBayesianmethod.Wealsobuiltall
assistanttooltoaddcolortasinsentencesforrapid
dataanddatainprocess.WithonthesearchresultPhospho.ELM,WeproposedamethodbasedBayesiandecisiontheoIy_PPSPtopredictthepotentialphosphorylationsitesofPKs。Predictionresultson~70PKgroupsshowthatingeneral,itoutperformsstateoftheartmethods:Scansite,KinasePhos,NetPhosKandGPS,whichsuggeststhatthismethodisanothercompetitivecomputationalapproachinthisbranchofbioinformaties.Atthesametime,thismethodhastheadvantageofsimpleness,efficiencyandrobustness.Awebserviceisalsoavailableforonlineperditionat(http://bioinformaties.1cd-ustc0rg/旦鳖).
3.Anovelmethodcalled“TranscriptionFactor-MediatedPathwayAnalysis’’ispresentedtoinferabnormaltranscriptionfactorsandpathwaysincancerchips.Theactivityofatranscriptionfactorisinferredbyevaluatingthenetresultofpercentsof
genesinaactivated(orrepressed)targetchip,andthentheabnormaltranscription
thefactorismappedtopathwaysdepositedinKEGGtThisalgorithmintegrates
experimentsofgene—regulationand
breastpathway.Wecancerhaveanalyzedhumangastriccancer,MicroarraycancerandIIdifferenttypesofwhichstoredinStanford
Database(sMD)in
pathwayarethismethodandfoundthatTGF-8,JAK-STAT,NF-?BandNotchover-activatedinmanyofthesechips.Theseabnormalpathwayswillbeofgreathelpinunderstandingtheprogressofcancerandinrationaldmgdesign.
TheresearchworkinthisdissertationissponsoredbytheKeyResearch
ofUniversityofProJeelsandScienceandTechnologyofChinaandDepartmentofScienceTechnologyofChina(Z004AAZ3511∞..IV-