信号通路相关文献挖掘与分析方法研究(4)

发布时间:2021-06-05

(recallrate93%andprecisionrate27%)witll-2%,theprecisionrateishighwith-18%enhanced.We

2.Proteinfoundabout63.000interestedarticleswiththismethod.importantroleintransforminginformationofkinases(PKs)play

signalpathway.Theyphosphorylatethesubstrates(proteins)atthespecificsites(phosphorylationsites)flankingwithcanonicalmotif.Onceagain,Weminedliterature

USedescribingphosphorylatesitesbyusingtheBayesianmethod.Wealsobuiltall

assistanttooltoaddcolortasinsentencesforrapid

dataanddatainprocess.WithonthesearchresultPhospho.ELM,WeproposedamethodbasedBayesiandecisiontheoIy_PPSPtopredictthepotentialphosphorylationsitesofPKs。Predictionresultson~70PKgroupsshowthatingeneral,itoutperformsstateoftheartmethods:Scansite,KinasePhos,NetPhosKandGPS,whichsuggeststhatthismethodisanothercompetitivecomputationalapproachinthisbranchofbioinformaties.Atthesametime,thismethodhastheadvantageofsimpleness,efficiencyandrobustness.Awebserviceisalsoavailableforonlineperditionat(http://bioinformaties.1cd-ustc0rg/旦鳖).

3.Anovelmethodcalled“TranscriptionFactor-MediatedPathwayAnalysis’’ispresentedtoinferabnormaltranscriptionfactorsandpathwaysincancerchips.Theactivityofatranscriptionfactorisinferredbyevaluatingthenetresultofpercentsof

genesinaactivated(orrepressed)targetchip,andthentheabnormaltranscription

thefactorismappedtopathwaysdepositedinKEGGtThisalgorithmintegrates

experimentsofgene—regulationand

breastpathway.Wecancerhaveanalyzedhumangastriccancer,MicroarraycancerandIIdifferenttypesofwhichstoredinStanford

Database(sMD)in

pathwayarethismethodandfoundthatTGF-8,JAK-STAT,NF-?BandNotchover-activatedinmanyofthesechips.Theseabnormalpathwayswillbeofgreathelpinunderstandingtheprogressofcancerandinrationaldmgdesign.

TheresearchworkinthisdissertationissponsoredbytheKeyResearch

ofUniversityofProJeelsandScienceandTechnologyofChinaandDepartmentofScienceTechnologyofChina(Z004AAZ3511∞..IV-

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