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2 Product description

2.1 The basic principle

NucleoZOL is designed for the isolation of total RNA (small and large RNA) in a single fraction or in separate fractions from a variety of sample materials, such as cells, tissue, and liquids from human or animal origin, plants, yeast, bacteria, viral materials, and other sources.

One of the most important factors during the isolation of RNA is to prevent degradation. First, cells and tissues are lysed and homogenized in NucleoZOL reagent based on guanidinium thiocyanate and phenol. Contaminating molecules such as DNA, polysaccharides, and proteins are precipitated by the addition of water and removed by centrifugation. The NucleoZOL procedure allows the separate isolation of small and large RNA by adding ethanol and isopropanol, respectively. RNA can be reconstituted by RNase-free water. A chloroform-induced phase separation is not necessary for high-quality RNA isolation.

The RNA is ready for use in qRT-PCR, microarrays, RNase protection assays, poly A+ isolation, blotting, and other applications.