UM_TotalRNA_NucleoZOL(3)

user's manuals about RNA or or

1.3 RNase-free work environment

The reagent has been tested for functionality. However, an RNase-free working environment is also a critical factor for performing successful RNA isolation and handling. Therefore, general recommendations to avoid RNase contamination should be followed:

Maintain a separate area, dedicated pipettors, and materials when working with RNA.

Wear gloves when handling RNA and reagents to avoid contact with skin, which is a source of RNases. Change gloves frequently.

Use sterile RNase-free plastic tubes. Tubes for lysate preparation and RNA precipitation have to be supplied by the user.

Keep all kit components sealed when not in use and all tubes tightly closed when possible.

1.4 About this user manual

Please read the detailed protocol if using NucleoZOL for the first time. Experienced users may refer to the short instruction manual.

All technical literature is available on the Internet at .

Please contact Technical Service regarding information about changes of to the current user manual compared to the previous revisions.