UM_TotalRNA_NucleoZOL(19)

user's manuals about RNA or or

Problem

Possible cause and suggestions

Volume of NucleoZOL used for homogenization was too low

Contamination with DNA

Increase volume of NucleoZOL.

Sample material contains strong buffers, organic solvents, alkaline solution, or salt

The precipitation of DNA (step 2) can be improved by the following modification: Increase incubation time to 15 min after addition of water (step 2). Centrifuge at 16,000 x e support protocol 6.1 for subsequent rDNase digestion in solution

Contamination with proteo-glycan, fat, or polysaccharide

Inefficient precipitation of contaminants

Centrifuge the initial crude homogenate (step 1) for phase separation in an additional step for 10 min at 12,000 x g.

6.3 Ordering information

ProductNucleoZOL

NucleoSpin® RNA Set for NucleoZOLNucleoSpin® RNANucleoSpin® RNA PlusNucleoSpin® RNA XS

NucleoSpin® RNA Clean-up XSNucleoSpin® RNA Clean-upRNase-free WaterElution Buffer AErDNase Set

REF740404.200740406.50

740955.10 / 50 / 250740984.10 / 50 / 250740902.10 / 50 / 250740903.10 / 50 / 250740948.10 / 50 / 250740378.1000740917.1740963

Pack of200 mL50 preps

10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps1000 mL1000 mLOne set