UM_TotalRNA_NucleoZOL(19)
时间:2025-07-11
时间:2025-07-11
user's manuals about RNA or or
Problem
Possible cause and suggestions
Volume of NucleoZOL used for homogenization was too low
Contamination with DNA
Increase volume of NucleoZOL.
Sample material contains strong buffers, organic solvents, alkaline solution, or salt
The precipitation of DNA (step 2) can be improved by the following modification: Increase incubation time to 15 min after addition of water (step 2). Centrifuge at 16,000 x e support protocol 6.1 for subsequent rDNase digestion in solution
Contamination with proteo-glycan, fat, or polysaccharide
Inefficient precipitation of contaminants
Centrifuge the initial crude homogenate (step 1) for phase separation in an additional step for 10 min at 12,000 x g.
6.3 Ordering information
ProductNucleoZOL
NucleoSpin® RNA Set for NucleoZOLNucleoSpin® RNANucleoSpin® RNA PlusNucleoSpin® RNA XS
NucleoSpin® RNA Clean-up XSNucleoSpin® RNA Clean-upRNase-free WaterElution Buffer AErDNase Set
REF740404.200740406.50
740955.10 / 50 / 250740984.10 / 50 / 250740902.10 / 50 / 250740903.10 / 50 / 250740948.10 / 50 / 250740378.1000740917.1740963
Pack of200 mL50 preps
10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps10 / 50 / 250 preps1000 mL1000 mLOne set