user's manuals about RNA or or

4

Bind RNA

For each preparation, take one NucleoSpin® RNA Column (light blue ring) placed in a Collection Tube and load 700 μL lysate. Centrifuge for 30 s at 8,000 x g.

Discard flow-through and reuse Collection Tube. Load residual lysate to the column and centrifuge for 30 s at 8,000 x g.

Discard Collection Tube with flow-through and place the column in a new Collection Tube (provided).

Maximum loading capacity of NucleoSpin® RNA Columns is 700 μL. Repeat the procedure if larger volumes are processed. 5

Wash and dry silica membraneAdd 700 μL Buffer RA3 to the NucleoSpin® RNA Column. Centrifuge for 30 s at 8,000 x g. Discard flow-through and reuse Collection Tube.Add 350 μL Buffer RA3 to the NucleoSpin® RNA Column. Centrifuge for 2 min at 8,000 x g.

Place the NucleoSpin® RNA Column into an RNase-free Collection Tube (1.5 mL, supplied). Open the lid of the column and let the membrane dry for 3 min.If the liquid level in the Collection Tube has reached the NucleoSpin® RNA Column after centrifugation, discard flow-through and centrifuge again.The procedure ensures complete removal of ethanol from the column.6

Elute RNA

Add 60 μL RNase-free H2O onto the center of the membrane and centrifuge for 1 min at 11,000 x g.