UM_TotalRNA_NucleoZOL(2)

user's manuals about RNA or or

1 Components

1.2 Reagents, consumables, and equipment to be supplied

by the user

Reagents

RNase-free water75 % ethanol70 % isopropanol100 % isopropanol

4-bromoanisole (optional, see p. 16)

1.5 mL, 2.0 mL or 15 mL centrifuge tubes (depending on the amount of sample to be processed per preparation) Sterile RNase-free tipsManual pipettorsVortex mixer

Centrifuge for microcentrifuge tubes

Equipment for sample disruption and homogenization Personal protection equipment (e.g., lab coat, gloves, goggles)Well ventilated working environmentRNase-free working environment

Consumables

Equipment