目的 探讨纤维化相关基因, 特别是与转化生长因子β(Transforming growth factor-β, TGF-β)信号通路相关的基因在人重度积水肾与正常肾组织中的差异表达变化。 方法 收集临床重度肾积水新鲜标本12例,正常肾组织标本6例,分别提取总RNA并纯化。选择TGF-β/骨形成蛋白(Bone

PCR-Microarray Analysis of Fibrotic Gene Expression in

Extensive Fibrotic Renal Tissue Resulting from

Hydronefrosis.

Yao Yi, Zhang Jie, Ye Dafu

Department of Urology, Renmin Hospital of Wuhan University. Wuhan（430060）

Abstract

Objective To investigate the fibrosis-associated genes, especially for that transforming growth factor-β (TGF-β) associated, expression in the tissue of extensive fibrotic kidney resulting from severe hydronephrosis. Methods Compared 12 extensive fibrotic kidneys resulting from severe hydronephrosis with 6 normal renal tissues using PCR array. All samples were collected from our hospital from Aug 2005 to Dec 2006. The data was treated with Ct Method. When 2 Ct ≤0.5 or ≥2, the difference was considered significant. Results A total of 49 differential genes expressed in the fibrotic renal tissues were screened out, of which, 25 were up-regulated and 24 were down-regulated. The differential genes included members and receptors of TGF-β super family and Bone morphogenetic protein (BMP) family, target molecular of TGF-β or BMP signal pathway and several regulator. Conclusion Through investigating with PCR array, we certificated the positive effect of Nodal, GSC and IGF1 to renal fibrosis. And we supposed that TGF-β play a secondary role in renal fibrosis, while BMP family and Lefty1 work in a functuional genome in the regulating of renal fibrosis. Keywords：Hydronephrosis; Renal fibrosis; Gene chip; Functional genomic

作者简介：姚颐，男，1980年生于湖北武汉，医学博士研究生在读，现就读于武汉大学人民医院泌尿外科，主要从事肾积水、肾间质纤维化病变的分子机制研究及临床治疗；