表达载体pHAT2图谱及序列信息(3)

表达载体pHAT2图谱及序列信息,该载体含有N-His标签

LOCUS pHAT2 4443 bp DNA circular 29-NOV-2006SOURCE ORGANISM COMMENT This file is created by Vector NTI /COMMENT ORIGDB|GenBankCOMMENT VNTDATE|428249734|COMMENT VNTDBDATE|428368939|COMMENT LSOWNER|COMMENT VNTNAME|pHAT2|COMMENT VNTAUTHORNAME|Hüseyin Besir|COMMENT VNTOAUTHORNAME|UNKNOWN|COMMENT VNTREPLTYPE|PlasmidCOMMENT VNTEXTCHREPL|BacteriaFEATURES Location/Qualifiers promoter 1..19 /gene="T7 prom" /vntifkey="30" /label=T7\prom /note="Promoter" terminator 219..347 /gene="T7 term" /vntifkey="43" /label=T7\term /note="Terminator" CDS complement(732..1814) /sequence="ORF_3 rf(5)" /vntifkey="4" /label=lacI promoter 2513..2541 /gene="amp prom" /vntifkey="30" /label=amp\prom /note="Promoter" CDS 2583..3443 /sequence="ORF_2 rf(3)" /vntifkey="4" /label=ampR /note="ampR" rep_origin 3598..4217 /gene="pBR322 origin" /vntifkey="33" /label=pBR322\origin /note="Rep_Origin" misc_binding 92..109 /vntifkey="20" /label=His-tagBASE COUNT 1097 a 1139 c 1144 g 1063 t ORIGIN 1 taatacgact cactataggg aattgtgagc ggataacaat tcctctagaa ataattttgt 61 ttaactttaa gaaggagata tatccatgag tcatcaccat caccatcact ccatggatat 121 cgaattcgtc gacctcgagg gatccgggcc ctctagatgc ggccgcatgc ataagcttga 181 gtattctata gtgtcaccta aatcccagct tgatccggct gctaacaaag cccgaaagga 241 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa 301 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggataac ctggcgtaat 361 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg