表达载体pHAT2图谱及序列信息(2)

表达载体pHAT2图谱及序列信息,该载体含有N-His标签

pHAT2

T7 promoter --> Lac operator XbaI

ATTAATACGACTCACTATAGGGAATTGTGAGCGGATAACAATTCCTCTAGAAATAATTTTTAATTATGCTGAGTGATATCCCTTAACACTCGCCTATTGTTAAGGAGATCTTTATTAAAA rbs His-tag NcoIATGCAAATTGAAATTCTTCCTCTATATAGGTACTCAGTAGTGGTAGTGGTAGTGAGGTACCTA METSerHisHisHsiHisHisHisSerMetAsp EcoRI SalI XhoI BamHI NotI NsiI HindIIIATCGAATTCGTCGACCTCGAGGGATCCGGGCCCTCTAGATGCGGCCGCATGCATAAGCTTTAGCTTAAGCAGCTGGAGCTCCCTAGGCCCGGGAGATCTACGCCGGCGTACGTATTCGAAIleGluPheValAspLeuGluGlySerGlyProSerArgCysGlyArgMetHisLysLeu <-- SP6 promoter

GAGTATTCTATAGTGTCACCTAAATCCCAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGCTCATAAGATATCACAGTGGATTTAGGGTCGAACTAGGCCGACGATTGTTTCGGGCTTTCGluTyrSerIleValSerProLysSerGlnLeuAspProAlaAlaAsnLysAlaArgLys111 NcoI 173 HindIII 1277 BstEII 2014 Eco56I122 EcoRI 264 EspI 1445 BclI 2016 NaeI128 SalI 264 Bpu1102I 1459 MluI 2196 NdeI134 XhoI 341 AccIII 1884 PflMI 2451 AatII136 SciI 341 BspMII 1894 Asp718I 2770 XmnI140 BamHI 650 BspMI 1894 Acc65I 2889 ScaI160 NotI 661 PstI 1898 KpnI 3304 Eco31I160 EagI 821 NarI 1902 Ecl136II 3370 Eam1105I160 Eco52I 822 EheI 1902 EcoICRI 3849 AlwNI160 XmaIII 824 BbeI 1904 SacI 4375 SapI171 NsiI 1048 BssHII 1904 SstIAflII AgeI AscI AsuII AvrII BalI

BglII BsmI Bsp1407I Bsu36I ClaI Csp45I

CspI CvnI DraIII Eco47III Eco72I EcoNI

FseI HpaI I-PpoI MfeI Mlu113I MscI

MstII NheI NruI PacI PinAI PmaCI

PmeI RleAI SacII SauI SfiI SgrAI

SmaI SnaBI SpeI SplI SpoI SrfISstII StuI SunI SwaI Tth111I XmaI