表达载体pHAT2图谱及序列信息

表达载体pHAT2图谱及序列信息,该载体含有N-His标签

EMBL Protein Expression and Purification Facility

pHAT2 - His-tagged expression vector

Features

T7 promoter

Lac operator

T7 gene 10 ribosome binding site

His-tag

Polylinker

SP6 promoter

T7 transcription terminator

LacI gene

β-Lactamase gene

Origin of replicationposition1-1721-4250-8092-109111-179184-202281-322732-1813 (reversed)2583-34333507-4203

Reference:

Peränen J., Rikkonen M.,Hyvönen M. & Kääriäinen L. (1996) T7 vectors with modified T7lac promoter forexpression of proteins in Escherichia coli. Anal. Biochem. 236

, 371-373.