胃癌组织中幽门螺杆菌cagA 和vacA 的表达及与其感染的相关性

胃癌组织中幽门螺杆菌cagA 和vacA 的表达及与其感染的相关性

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8日; 14(26): 2632-2635世界华人消化杂志 2006年9月18

ISSN 1009-3079 CN 14-1260/R

临床经验 CLINICAL PRACTICE

胃癌组织中幽门螺杆菌cagA和vacA的表达及与其感染的相关性

佟书娟, 陈 军, 詹 瑧, 杨丹丹, 刘亚平

■背景资料

胃癌的发生是一个多因素、多阶段和多基因的演变过程, 微生物、寄生虫等生物致病因子是导致胃癌形成的重要病因, 研究发现, 生物致病因子幽门螺杆菌(H pylori)感染与胃癌的发生密切相关.

佟书娟, 詹瑧, 杨丹丹, 南京中医药大学基础医学院 江苏省南京市 210029

陈军, 中国人民解放军宁夏军区政治部 宁夏回族自治区银川市 750021

刘亚平, 中国人民解放军第5医院病理科 宁夏回族自治区银川市 750004

宁夏教育厅科研基金资助项目, No. JY2002125

通 佟书娟, 210029, 江苏省南京市, 南京中医药大学基础医学院. tongshujuan6@http://www.77cn.com.cn电话: 025-85811925收稿日期:: 2006-05-2 2006-05-2 日期:2006-05-2 日期:: 2006-08-102006-08-10

Expression of Helicobacter pylori cagA and vacA and their correlations with Helicobacter pylori infection in gastric cancer

Shu-Juan Tong, Jun Chen, Zhen Zhan, Dan-Dan Yang, Ya-Ping Liu

Shu-Juan Medical College, Nanjing University of Traditional Chinese Tong, Zhen Zhan, Dan-Dan Yang, Basic Medicine, Nanjing 210029, Jiangsu Province, China

Jun Chen,mand of Chinese PLA, Yinchuan 750021, Ningxia Hui Au- Department of Politics, Ningxia Military Com-tonomous Region, China

Ya-Ping Liu,Chinese PLA, Yinchuan 750004, Ningxia Hui Autonomous Department of Pathology, the Fifth Hospital of Region, China

Supported partment of Education of Ningxia Hui Autonomous Region, by the Science Research Foundation from De-No. JY2002125

Correspondence lege, to: Shu-Juan Tong, Basic Medical Nanjing 210029, Jiangsu Province, Nanjing University of Traditional Chinese Medicine, Col-China. tongshujuan6@http://www.77cn.com.cn

Received: 2006-05-23 Accepted: 2006-08-10

Abstract

AIM:and vacA To explore the expression of in gastric cancer and their correlations H pylori cagA with H pylori infection.

METHODS: by Warthin-Starry silver staining in gastric can-H pylori infection was determined cer ((merase chain reaction (PCR) was used to detect n = n24) = 39), gastric mucosal atypical hyperplasia and chronic gastritis (n = 33). Poly-the expression of H pylori cagA and vacA.

RESULTS: H pyloriThe infection rates of H pylori, cagA+ sues were significantly higher than those chronic and vacA+ H pylori in gastric cancer tis-gastritis tissue (< of 0.05; χ2 = 7.00, P < 0.05; χ2 = 15.20, P H pyloriχ2 = , cagA12.43, + H pyloriP < 0.05); and the vacAinfection +rates gastric mucosal atypical hyperplasia were mark- H pylori in edly higher P < 0.05; than those in chronic gastritis (χ2 = 11.04, P < 0.05; χ2χ2= 6.25, PH < higher in poorly-differentiated gastric cancer in pylori0.05). and The vacAinfection = 11.61, + H pylorirates were of H significantly pylori, cagA+ comparison with those in moderately- and well-differentiated one (χ2 = 8.19, P < 0.05; χ2Pwas positively related to < 0.05; = 13.14, χ2 = 6.62, P < 0.05). expression H pyloriH pylori infection atypical hyperplasia and gastric cancer (chronic in chronic gastritis, gastric cagA and mucosal vacA gastritis: atypical hyperplasia: r = 0.56, P < 0.01; gastric cancer: r< = 0.64, 0.01; rP < 0.01; = 0.64, P < 0.01; P < 0.01).

r = 0.90, P < 0.01; r = 0.92, r = 0.95, P CONCLUSION: tant H pylori gastritis promoter cancer, into atypical in the development infection is an impor-hyperplasia and of chronic gastric and and it may accelerate the proliferation inducing injury of gastric mucosa epithelial cells by Their roles in the pathogenesis of gastric cancer.

synergistic cagA and vacAeffects expression, respectively. may play important Key Words: Gastric cancer; Helicobacter pylori; Cytotoxin associated gene A; Vacuolating cytotoxin gene A

Tong SJ, Chen J, Zhan Z, Yang DD, Liu YP. Expression of with Helicobacter pylori cagA and vacA and their correlations Huaren Xiaohua Zazhi 2006;14(26):2632-2635

Helicobacter pylori infection in gastric cancer. Shijie 摘要

目的: 研究H pylori cagA和H pylori vacA在胃癌、胃黏膜不典型增生和胃炎组织中的表达及与H pylori感染的相关性.

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胃癌组织中幽门螺杆菌cagA 和vacA 的表达及与其感染的相关性

佟书娟, 等. 胃癌组织中幽门螺杆菌cagA和vacA的表达及与其感染的相关性 2633

方法: 采用Warthin-Starry嗜银染色法检测胃癌组织39例, 胃黏膜不典型增生组织24例和慢性胃炎组织33例中H pylori感染情况; PCR法检测上述标本中H pylori cagA和H pylori vacA的表达.

结果: 胃癌组织中H pylori, H pylori cagA+株和H pylori vacA+株感染率显著高于慢性胃2P2P情况; 采用PCR法检测上述标本中细胞毒素相关基因A和空泡细胞毒素基因A的表达, 探讨H

pylori感染、细胞毒素相关基因A和空泡细胞毒素基因A表达与胃癌发生的相关性及意义. 1 材料和方法

1.1 材料 解放军第5医院2002-01/2003-12纤维内镜活检存档蜡块标本96例, 胃癌39例, 男32例, 目前, H pylori研究的热点为H pylori的毒力因子cagA和vacA与胃癌发生的关系及对致癌物形成的影响, 胃黏膜H pylori感染与上皮细胞增殖、细胞凋亡的关系, H pylori感染对胃黏■研发前沿

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