3期郭亭亭等:昆虫细胞色素P450基因的多样性、进化及表达调控

表1 已克隆的昆虫P450基因cDNATable1 ClonedP450cDNAininsects

303

片段数

种类Species

cDNA全长序列cDNAful-llengthsequences

Numberofsegments

鳞翅目Lepidoptera家蚕Bombyxmori

CYP4G25,

CYP4M5,

CYP4M9,

CYP6AB4,

CYP6AB5,

CYP6AE,

CYP6AE2,

CYP6AE9,

20

CYP6AE8,CYP6AE22,CYP6AE21,CYP6AE7,CYP6AU1,CYP6B29,CYP9A20,CYP9A19,CYP9A19(strainDazao),CYP305B1,CYP337A1

小菜蛾Plutellaxylostella野桑蚕Bombyxmandarina烟芽夜蛾Heliothisvirescens烟草天蛾Manducasexta斜纹夜蛾Spodopteralitura香芹黑凤蝶Papiliopolyxenes天蚕Antheraeayamamai欧洲防风草结网毛虫Depressariapastinacella棉铃虫Helicoverpaarmigera美洲棉铃虫Helicoverpazea凤蝶Papiliocanadensis北美黑条黄凤蝶Papilioglaucus甘蓝夜蛾Mamestrabrassicae烟青虫Helicoverpaassulta小计Subtotal双翅目Diptera黑腹果蝇

Drosophilamelanogaster

CYP4AC1,CYP4AC2,CYP4D8,CYP4G15A,CYP6A9,CYP6A22,

CYP4D14,CYP6A13,CYP6A23,

CYP4AC3,CYP4AD1,CYP4D21,

CYP4P1,CYP6A14,CYP6D2,

CYP4E1,CYP4P2,CYP6A17,CYP6D4,

CYP4C3,CYP4E2,CYP4P3,CYP6A18,CYP6D5A,

CYP4D1A,CYP4D1B,CYP4D2,CYP4E3,CYP4S3,CYP6A19,CYP6D5B,CYP18,

CYP4G1,CYP6A2,CYP6A20,CYP6G1,

CYP4AE1,CYP6A8,CYP6A21,CYP6G2,CYP18A1B,CYP311A1B,CYP313B1,

59

CYPM3R9,

CYPM3R5,

CYP6AA1,

CYP6M1,

CYP6N1,

CYP6N2,

CYP6P1,

CYP6P2,

162

CYP6BF1(v1,v2,v4)

CYP4M9,CYP6AB4,CYP6B29,CYP9A19,CYP9A22,CYP305B1v1,CYP9A1v2

CYP4M2,CYP307A1,CYP314A1,CYP315A1,CYP302A1,CYP306A1

CYP4M14v1,CYP4S9v1

CYP6B3(v5,v4,v3c,v3b,v3a),CYP6B1(v2,v1)CYP4G25

CYP6AB3v2,CYP6AB7,CYP6AB3,CYP6AE1C

CYP6AE12,CYP6B2,CYP6B6(midgut),CYP6B6(fatbody)CYP6B7,CYP9A12,CYP9A14,CYP9A17,CYP9A18

CYP4M6,CYP4M7,CYP6B8,CYP321A1CYP6B25,CYP6B26

CYP6B4,CYP6B23,CYP6B24CYP4L4,CYP4S4178

84109811914012250110

CYP9A19(strainLaos),CYP9A22,CYP9A22(strainDazao),

CYP18A1,

CYP302A1,

CYP315A1,

CYP306A1,

CYP9A22(strainLaos),CYP9A21,

CYP4G15B,

CYP6T1,CYP6T3,CYP6U1,CYP6V1,CYP6W1,CYP9B1,CYP9B2,CYP9C1,CYP9F2,CYP9H1,CYP12A4,CYP12A5,CYP307A1,CYP308A1,CYP312A1,

拟暗果蝇Drosophilapseudoobscura冈比亚按蚊Anophelesgambiae

CYP313A1,

CYP12B2,CYP12C1,

CYP309A2,CYP313A3,

CYP18A1A,CYP311A1A,CYP313A5,

CYP28A5,CYP28C1,CYP28D1,CYP28D2,CYP301A1,CYP303A1,CYP304A1,CYP305A1,

CYP309A1,CYP313A2,

CYP310A1,CYP313A4,

CYP314A1,CYP315A1,CYP316A1,CYP317A1,CYP318A1

CYP6P3,CYP6P4,CYP6R1,CYP6S1,CYP6S2,CYP6Y1,CYP6Z1,CYP6Z2,CYP6Z3,CYP9K1,CYP12F1,CYP12F2,CYP12F3,CYP12F4CYP4D3v2,

家蝇Muscadomestica

CYP4D4v2,

CYP4D35,

CYP4D36,

CYP4G2v1,

CYP4G13v2,

CYP6A5v2,

CYP6A24,CYP6A36,CYP6A37,CYP6A38v1,CYP6D1,CYP6D3v2,CYP6D3v3,CYP12A1,CYP12A2,CYP28B1

5