异丙酚对肝脏缺血再灌注损伤时的保护作用

Effect of propofol on hepatic ischemia reperfusion in rats

An Shi-zhi,Wang Yue-lan,Guo Hong-yan.Department of Anesthesiology,Jinan women and children hospital,Master of Shandong University,Jinan 250001,Shandong,China

Abstract

[Objective] Hepatic injury caused by ischemia and reperfusion(HIRI)is a very important problem in clinical practice.It is related to the exclusion of liver transplantation and hepatic failure,which caused by shock, infection, trauma of liver and liver transplantation. HIRI effects revival of liver and recovery of hepatic function after hepatectomy.A large number of researches testified that ,some factor participate in the HIRI,such as increase of free radical intracellular, calcium overload, cytokines, kupffer cell (KC) activation and polymorphonuclear leukocyte(PMN)aggregation and so on,but the mechanism and its pathway are not clear.

This study aimed to observe the mechanism of I/R and the effects of propofol in rat in vivo from molecularbiology to morphology ，from cytokines to NF- kB activation.Which proved the theory foundation for reducing perioperative HIRI and finding out rational anesthetic.

[Methods] First of all to build the model of I/R for rat according to the method of Kobayashi,and then seventy and two healthy Wistar rats were randomly divided into the nomal control group(A),ischemia and reperfusion group(B),I/R+propofol 4mg.h-1.100g-1 (C)and 6mg.h-1.100g-1(D)group.Blood plasma and partial hepatic issue were obtained at 30min-ischemain and 30min-reperfusion(T1),at 60min-ischemain and 60min(T2)、at 60min-ischemain and 120min-reperfusion(T3). Blood plasma levels of TNF- a and IL-10 were measured with ELISA.The NF-kB activation to special DNA sequence were measured with EMSA.The ultramicrostructure ,cell apoptosis and mitochondria energy charge were observed by electron microscope.

[Results]1.Blood plasma TNF-a and IL-10 levels of T1、 T2、 T3 significantly increased compared with group A(P＜0.05)in group B , but reached peek at 3