正交试验法优选通络颗粒剂的提取工艺

extracting time were screened by L9(34) orthogonal test, Alcohol-extracting fraction：with the weight of alcohol-extracting fraction and hesperidin content as the indexes, alcohol concentration, extracting times, alcohol volume and extracting time were screened by L9(34) orthogonal test. Results The optimal extraction conditions were as follows：water-extracting fraction：extracting 3 times with 12-fold water, 1.5 hours for each time；alcohol-extracting fraction：refluxing and extracting 2 times with 10-fold 60% alcohol, 1.5 hours for each time. Conclusion The results can provide theoretical basis for production of Tongluo granule.

Key words：Tongluo granule；paeoniflorin；hesperidin；orthogonal design

通络颗粒剂由赤芍、枳实、丹参、三七、黄芪等药加工而成,具有疏肝理气、化瘀通络的功能,用于肝郁气滞、瘀血内结、胞脉不通证,主治慢性子宫内膜炎、输卵管炎、盆腔炎性包块及盆腔粘连等。处方中部分中药含有脂溶性有效成分,部分中药含有水溶性有效成分。为此,本实验用水提取黄芪、赤芍、柴胡、甘草等,用乙醇提取枳实、丹参等,采用正交设计法对提取工艺条件进行优选。

1 仪器与试药