抗黄瓜花叶病毒RNAi载体的构建及烟草的转化(4)

时间:2025-03-12

72AgriculturalScience&TechnologyVol.11,No.5,2010

[10]

.Theconstructedexprestheefficiencyofgenesilencing

icotianatosionvectorpBi35SCR2wastransformedintotheN

baccowithAgrobacteriummediatedmethod,theobtainedtransgenictobaccoplantscouldbeobservedontheantiviralabilityoftransgenictobaccoplantsbychallengetest,ifthe

,itcouldbefurtherappliedtotomatoproeffectwasobvious

cessing,whichhadlaidthefoundationfortheantiCMVprocessingtomatoplants.

M:DL2000;1:pBi35SCR2vectorcontrol;2-5:pBi35SCR2vec

tortransgenictobaccolines;6:PBI121transgenictobacco.Fig.7 PCRidentificationofCR2_BKfragmentintransgenicto

baccoplant

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