抗黄瓜花叶病毒RNAi载体的构建及烟草的转化(4)
发布时间:2021-06-05
发布时间:2021-06-05
72AgriculturalScience&TechnologyVol.11,No.5,2010
[10]
.Theconstructedexprestheefficiencyofgenesilencing
icotianatosionvectorpBi35SCR2wastransformedintotheN
baccowithAgrobacteriummediatedmethod,theobtainedtransgenictobaccoplantscouldbeobservedontheantiviralabilityoftransgenictobaccoplantsbychallengetest,ifthe
,itcouldbefurtherappliedtotomatoproeffectwasobvious
cessing,whichhadlaidthefoundationfortheantiCMVprocessingtomatoplants.
M:DL2000;1:pBi35SCR2vectorcontrol;2-5:pBi35SCR2vec
tortransgenictobaccolines;6:PBI121transgenictobacco.Fig.7 PCRidentificationofCR2_BKfragmentintransgenicto
baccoplant
s
References
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M:DL2000;1:pBi35SCR2vectorcontrol;2-5:pBi35SCR2vec
tortransgenictobaccolines;6:PBI121transgenictobacco.Fig.8 PCRidentificationofCR2_SBfragmentintransgenicto
baccoplants
Discussions
TheCMVRAN2genomesequenceofCMVNS04isolates
,andthefunctionalgenesequenceawasclonedinthisstudy
lignmentandphylogeneticanalysisshowedthatthecucumbermosaicvirusCMVNS04isolatesofXinjianghadahighernucleotideandaminoacidhomologywithDQ412731isolates
,Zhejiang,whichreached98.0%and96.5%refromChina
spectively.Inthisstudy,theconservedsequencesinCMVRNA2genomewhichwaslessstudiedwasselectedasthetar
,andvectorwouldformhpRNAgetsequenceofRNAivector
structureaftertranscription,andthehpRNAstructureincluded
,andtherewasaringstructureofasenseandantisensearms
about50bpbetweenthetwoarms,whichhelpedtoimprove
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NAi摘要)[&'\]^_R`a1bcUde1fg(
张瑜,郑银英,赵峰玉,乔亚红,崔百明,向本春 (ãä+X%åæ3 z¦§¨©,ãä+X%3 %&,âçãä
32003)+8
[NAi41]èéê>?ë{ìíRîï,«Õîïðñòó。[T-PCRõÂ,S04NA2MVRAN256]ô Rö÷>?ë{ìíNø$ùú¢ª 5Rûüý5ÐÑ。ÈþCûüýs5ÿ!"#$ L
Bi35SCR2CR5õÂÃÄîï ,%ÐÑ,èép¶&º'îï,«=º'îï()¬G;½¾åZ4*+5õÂÕº'îïðñòó, P
ðñ。[RNA2MV2aQ412731¢ª ~6w&78n9û87 :,78]-. #¸¢Ò/ º0,s12C5ÐÑ s3455D¢;'¼98.0%[96.5%;PCR/ º0,Bi35SCR2<¨=>èéÜp¶&º'îï,«=>Õº'îïðñòó。[],79<¨É»5ðûüòóÊ L?@Aí<¨5B «L ø$ùúê>?ë{ìíCGÜûD。
RNAi:;< >?ë{ìí;7 :;îï;òó;ð#
2008DFA30560);973计划前期研究专项(2008CB117018);石河子大学高层次人才科研启动项目(RCZX200732)。XYZ4 国际科技合作项目(
1984-),女,黑龙江哈尔滨人,硕士研究生,研究方向:植物基因工程。通讯作者,教授,博士生导师。=>?@ 张瑜(0100526 DEBC 20100629-ABC 2