Flow-Cytometric Immune Function Methodology(17)

Flow-Cytometric Immune Function 57

development as well as interferon-γ production by T helper 1 cells. Eur. J. Immunol.26, 659–668.

30. Lapointe, R., Toso, J. F., Butts, H. A., Young, H. A., and Hwu, P. (2000) Human

dendritic cells require multiple activation signals for the ef cient generation of tumor antigen-speci c T lymphocytes. Eur. J. Immunol.30, 3291–3298.

31. Zhou, L.-J. and Tedder, T. F. (1995) A distinct pattern of cytokine gene expression

by human CD83+ blood dendritic cells. Blood86, 3295–3301.

32. Nomura, L. E., Walker, J. M., and Maecker, H. T. (2000) Optimization of whole

blood antigen-speci c cytokine assays for CD4+ T cells. Cytometry40, 60–68. 33. Suni, M. A., Picker, L. J., and Maino, V. C. (1998) Detection of antigen-speci c

T cell cytokine expression in whole blood by ow cytometry. J. Immunol. Methods212, 89–98.

34. Waldrop, S. L., Pitcher, C. J., Peterson, D. M., Maino, V. C., and Picker, L. J. (1997)

Determination of antigen-speci c memory/effector CD4+ T cell frequencies by ow cytometry. J. Clin. Invest.99, 1739–1750.

35. Jung, T., Schauer, U., Heusser, C., Neumann, C., and Rieger, C. (1993) Detection

of intracellular cytokines by ow cytometry. J. Immunol. Methods159, 197–207. 36. Picker, L. J., Singh, M. K., Zdraveski, Z., Treer, J. R., Waldrop, S. L., Bergstresser,

P. R., et al. (1995) Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by ow cytometry. Blood86, 1408–1419.

37. De Caestecker, M. P., Telfer, B. A., Hutchinson, I. V., and Ballardie, F. W.

(1992) The detection of intracytoplasmic interleukin-1α, interleukin-β, and tumour necrosis factor a expression in human monocytes using two colour immuno uorescence ow cytometry. J. Immunol Methods154, 11–20.

38. Zhou, L.-J. and Tedder, T. F. (1995) Human blood dendritic cells selectively

express CD83, a member of the immunoglobulin superfamily. J. Immunol.154,3821–3835.