自噬与核糖体的关系

dilutionsweremadeinsterilewaterand3μLofeachdilutionwasspottedontoYPDplatesintheabsenceorpresenceof1ng/mLrapa-mycin.Plateswereincubatedasindicated.

ACKNOWLEDGMENTS

TheauthorsthankJeffColler,JonathanWeissman,RoyParker,TedPowers,andDieterWolfeforprovidingstrainsandplasmids,andMichaelAltmann,TerriKinzy,andTomDevorforprovidinganti-sera.TheauthorsalsothankKimKeelingforprovidingcriticalcom-mentsonthemanuscript.ThisworkwassupportedbyNationalInstitutesofHealth(NIH)R01grantGM068854(toD.M.B.).ReceivedMarch12,2014;acceptedJanuary5,2015.

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