常见酶切位点及其保护碱基

常用酶切位点的保护性碱基Enzyme Oligo Sequence GGTCGACC CGGTCGACCG CCGGTCGACCGG CACATGTG CCACATGTGG CCCACATGTGGG GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA CGGATCCG CGGGATCCCG CGCGGATCCGCG CAGATCTG GAAGATCTTC GGAAGATCTTCC GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA GGGT(A/T)ACCC AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG GGAATTCC CGGAATTCCG CCGGAATTCCGG GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG GGGTACCC GGGGTACCCC CGGGGTACCCCG GACGCGTC CGACGCGTCG CCCATGGG CATGCCATGGCATG CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG TTGCGGCCGCAA Chain Length 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 9 22 24 27 8 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 8 14 8 10 12 18 20 22 8 10 12 12 % Cleavage 2 hr 20 hr 0 0 0 0 0 0 0 0 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 50 >90 >90 >90 >90 >90 10 25 >90 >90 >90 >90 0 0 75 >90 25 >90 0 0 0 0 50 >90 0 10 0 0 25 50 25 >90 0 0 0 0 >90 >90 50 50 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 0 0 0 0 10 75 0 0 >90 >90 >90 >90 0 0 25 50 0 0 50 75 0 0 0 0 0 0 0 0 75 >90 75 >90 0 0 10 25 10 50 0 0

AccI

AflIII

AscI

AvaI

BamHI

BglII

BssHII BstEII BstXI

ClaI

EcoRI

HaeIII

HindIII

KpnI

MluI NcoI

NdeI

NheI

NotI

NsiI

PacI

PmeI

PstI

PvuI SacI SacII

SalI

ScaI

SmaI

SpeI

SphI

StuI

XbaI

XhoI

XmaI

ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT TTAATTAA GTTAATTAAC CCTTAATTAAGG GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT CCGATCGG ATCGATCGAT TCGCGATCGCGA CGAGCTCG GCCGCGGC TCCCCGCGGGGA GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCG ACGCGTCGACGTCGGCCATAGCGGCCGCG GAA GAGTACTC AAAAGTACTTTT CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG GGCATGCC CATGCATGCATG ACATGCATGCATGT AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA

16 20 24 28 12 22 8 10 12 8 10 12 24 8 14 22 24 26 8 10 12 8 8 12 28 30 32 8 12 6 8 10 12 8 10 12 14 8 12 14 8 10 12 8 10 12 14 8 10 12 8 10 12 14

10 10 25 25 10 >90 0 0 0 0 0 0 75 0 10 >90 >90 0 0 10 0 10 0 50 0 10 10 10 75 0 0 10 >90 10 10 0 0 0 0 10 >90 >90 >90 0 >90 75 75 0 10 10 0 25 50 >90

10 10 90 >90 >90 >90 0 25 >90 0 25 50 >90 0 10 >90 >90 0 0 25 10 10 0 >90 0 50 75 25 75 10 10 50 >90 >90 >90 50 50 0 25 50 >90 >90 >90 0 >90 >90 >90 0 25 75 0 75 >90 >90