pET28a-c(+)质粒图谱

pET28a(+),pET28b(+),pET28c(+)的质粒图谱,希望对大家有用

pET-28a-c(+) VectorspET-28a DNA pET-28b DNA pET-28c DNA Cat. No. 69864-3 69865-3 69866-3

TB074 12/98 The pET-28a-c(+) vectors carry an N-terminal His Tag®/thrombin/T7 Tag® con guration plus an optional C-terminal His Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

Call 137-649-32037 to buy the vector in China.

pET-28a(+) sequence landmarks

Pvu I(4426) Sgf I(4426) Sma I(4300)

Kan ( 399 5-4 80 7)

T7 promoter T7 transcription start His Tag coding sequence T7 Tag coding sequence Multiple cloning sites (BamH I - Xho I) His Tag coding sequence T7 terminator lacI coding sequence pBR322 origin Kan coding sequence f1 origin

370-386 369 270-287 207-239Dra III(5127)

Bpu1102 I(80)

Xho I(158) Not I(166) Eag I(166) Hind III(173) Sal I(179) Sac I(190) EcoR I(192) BamH I(198) Nhe I(231) Nde I(238) Nco I(296) Xba I(335) Bgl II(401) SgrA I(442) Sph I(598)

158-203 140-157 26-72 773-1852 3286 3995-4807 4903-5358

5358) 4903in ( rig o f1

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.

Mlu I(1123) Bcl I(1137) BstE II(1304) Apa I(1334) BssH II(1534) EcoR V(1573) Hpa I(1629)

73-1852) lacI (7

Cla I(4117) Nru I(4083)

pET-28a(+)(5369bp)Eco57 I(3772) AlwN I(3640)

i orBssS I(3397)

(3 2

86 )

PshA I(1968) Bgl I(2187) Fsp I(2205) Psp5 II(2230)

BspLU11 I(3224) Sap I(3108) Bst1107 I(2995) Tth111 I(2969)

T7 promoter primer#69348-3 pET upstream primer#69214-3 Bgl II T7 promoter His Tag lac operator

AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGA TATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAA MetGlySerSerHisHisHisHisHisHisSerSerGlyLeuValProArgGlySerHisMetAlaSerMetThrGlyGlyGlnGln ATGGGTCGCGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCC MetGlyArgGlySerGluPheGluLeuArgArgGlnAlaCysGlyArgThrArgAlaProProProProProLeuArgSerGlyCysEnd ...GGTCGGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCC ...GlyArgAspProAsnSerSerSerValAspLysLeuAlaAlaAlaLeuGluHisHisHisHisHisHisEndBamH I EcoR I Sac I Sal I Hind III Eag I Not I Xho Ithrombin His Tag

Xba I

rbs T7 Tag

Nco I

Nde I Nhe I

pET-28a(+) pET-28b(+)

...GGTCGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCA

CTGAGATCCGGCTGCTAACAAAGCCC pET-28c(+) ...GlyArgIleArgIleArgAlaProSerThrSerLeuArgProHisSerSerThrThrThrThrThrThrGluIleArgLeuLeuThrLysPro... GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGT7 terminator primer#69337-3

Bpu1102 I

T7 terminator

pET-28a-c(+) cloning/expression regionNovagen ORDERING 800-526-7319 TECHNICAL SUPPORT

800-207-0144

pET28a(+),pET28b(+),pET28c(+)的质粒图谱,希望对大家有用

pET-28a(+) Restriction Sites

TB074 12/98

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