Patterns of Protein Synthesis and Tolerance of Anoxia in Root

312Changetal.PlantPhysiol.Vol.122,2000

TableI.Continued

DifferencefromCalculatedMass 0.010.000.000.000.010.040.040.040.020.070.05 0.030.060.06 0.050.070.060.070.09

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCoveredHomologoustoMetsynthase

C.roseus(X83499)23%

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic36.8

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kD84.9/77

pI6.10/6.17

35

629.29684.40734.46812.501,021.561,041.581,096.621,470.791,470.791,658.901,791.021,807.021,807.021,848.941,848.941,977.051,991.072,310.372,438.49

36

796.40976.581,053.591,104.671,114.701,156.621,366.611,461.891,553.791,570.791,579.871,587.761,800.821,827.992,059.152,075.122,911.53 0.010.020.020.010.010.030.010.040.060.030.030.050.070.050.060.030.04

(R)NDQPR(F)(R)EGLPLR(K)(K)(R)EGLPLRK(A)(K)SWLAFAAQK(V)

( )AcetN-ASHIVGYPR(M)(K)YLFAGVVDGR(N)

( )AcetN-ASHIVGYPRMet-oxGPK(R)(R)FETCYQIALAIK(D)

(K)(K)GMLTGPVTILNWSFVR(N)(K)ILTALKGVTGFGFDLVR(G)

(K)GMet-oxLTGPVTILNWSFVR(N)(K)AEHAFYLDWAVHSFR(I)(R)KYAEVKPALENMVSAAK(L)(R)KAEHAFYLDWAVHSFR(I)(K)LQEELDIDVLVHGEPER(N)(K)LNLPVLPTTTIGSFPQTLELR(R)(K)KLNLPVLPTTTIGSFPQTLELR(R)

1,100.5817,1,293.66,1,326.64,1,517.86,1,555.76,1,745.03,1,830.03,1,957.08,2,087.98,2,103.98,2,124.11,2,178.13,2,268.19

(R)VFDMet-oxLR(R)(K)GVAINFVTR(D)

(R)pyroGluSLRPDNIK(M)(R)KGVAINFVTR(D)(R)VLITTDLLAR(G)(K)VHACVGGTSVR(E)

( )AcetN-AGMet-oxAPEGSQFDAK(H)(R)(V)(K)pyroGluFYVNVDKEDWK(L)(K)QFYVNVDKEDWK(L)(K)DQIYDIFQLLPSK(I)

(K)Met-oxFVLDEADEMet-oxLSR(G)(R)DHTVSATHGDMet-oxDQNTR(D)(R)(G)(K)IQVGVFSATMPPEALEITR(K)

(K)IQVGVFSATMet-oxPPEALEITR(K)

(R)GIDVQQVSLVINYDLPTQPENYLHR(I)1,563.87,2,239.10,2,807.32

(R)VHILTDGR(D)(R)LDQLQLLLK(G)(R)YLVSPPEIDR(T)(K)IYDGDGFNYIK(E)

(R)YAGMet-oxLQYDGELK(L)(R)GWDAQVLGEAPYK(F)(K)RGWDAQVLGEAPYK(F)

(R)MVMLAKALEYADFDNFDRVR(V)861.07,1,030.11,1,066.07,1,101.58,1,165.57,1,280.64,1,320.60,1,365.65,1,475.67,1,482.71(R)QIFDSR(G)(K)YNQLLR(I)

(R)IEEELGAIAVYAGAK(F)(R)IPLYQHIANLAGNK(Q)

Translationalinitiationfactor4A

Z.mays(U73459)56%

ConsistentwithWestern-blotresult

47.0/495.38/5.7535.7

3738

910.491,083.671,188.641,304.621,403.651,433.691,589.842,420.27 0.020.000.010.000.00 0.010.030.10

Notidentified

2,3-Bisphosphoglycerate-independentphosphoglyceratemutase

Z.mays(M80912)15%

N.A./6260.6/63N.A./6.25.29/5.9135.434.6

39

765.38806.451,533.871,551.87 0.010.000.050.01ENO2Z.mays(U17973)36%

48.1/535.71/6.0334.5