膜联蛋白A11对人胃癌细胞株SGC7901氟尿嘧啶耐药性的影响及机制

膜联蛋白A11对人胃癌细胞株SGC7901氟尿嘧啶耐药性

的影响及机制

贾楠;李勇;赵一杰;赵群;范立侨;檀碧波;刘羽

【期刊名称】《中华实验外科杂志》

【年(卷),期】2018(035)004

【摘要】Objective To investigate the effect of Annexin A11 (ANXA11) on 5-fluorouracil (5-Fu) resistance of human gastric cancer cell line SGC7901.Methods The expression of ANXA11 in human gastric cancer cell lines SCG7901 and BGC823 was detected.ANXA11-small interfering RNA (siRNA) and negative control sequences were transiently transfected into SGC7901 cells.cell counting kit-8 (CCK-8) assay was used to detect the sensitivity to 5-Fu with different dose (1,10,20,40,80 μg/ml) in each group.Apoptosis rate of each group was detected by flow cytometry.Quantitative polymerase chain reaction (qPCR) and Western blotting were used to detect the changes of B cell lymphoma/ leukemia-2 associated X protein (bax),B cell lymphoma/leukemia-2 (bcl-2) and TS.Results Compared with the BGC-823 cells,ANXA1 1 mRNA and protein expressions in SGC7901 cells were significantly higher (t =3.212,P =0.033;t =-10.768,P =0.000).50％ inhibitory dose (IC50) and 20％inhibitory dose (IC20) of the transfection group (transfected with ANXA11-siRNA) was significantly lower than the negative control (transfected with NS-siRNA) and blank control group (F =47.500,P