Cells

yaDLipofectamine® LTX Reagent

DNA and Plus Reagent

1 yaD

4–2 yaD

Plasmid Transfection

Transfect cells according to the following table. The transfection is designed for 1 DNA and PLUS reagent amount combined with 4 different amounts of Lipofectamine® LTX. For each lipid reagent amount, the prepared mix is enough to have triplicates (96-well), duplicates (24-well), and single well

(6-well) transfections, and account for pipetting variations.

The following table shows the amounts of DNA and Lipofectamine® LTX Reagent per well used in each transfection reaction. For additional information on scaling your transfection reaction, see page 4.