蛋白质常用buffer

实验室常用溶液配方

PBS(1×)(1L) 8.0g NaCl

0.2g KCl

1.44g Na2HPO4

(3.6283g Na2HPO4.12H2O

2.716g Na2HPO4.7H2O)

0.24g KH2PO4

80ml H2O

调至pH 7.4, add Milli-Q to 1L

Running Buffer(1×)(1L)

3g Tris

14.4g Gly

1g SDS

Add ddH2O to 1L

Transfer Buffer(1×)(1L)

5,8g Tris

2.9g Gly

0.37 SDS

200ml Methanol

Add ddH2O to 1L

TBE (5×)(1L)

54g Tris

27.5g Boric acid

20ml 0.5M EDTA (pH 8.0)

Add ddH2O to 1L

NP40 Lysis Buffer

5ml 3M NaCl

2.5ml 1M Tris-HCl (pH 6.8)

2.5ml 1M Tris-HCl (pH 8.0)

1ml NP40

Add ddH2O to 100ml