生化英文教学大纲

Biochemistry Course Outline

Preface

Biochemistry and molecular biology focus on the chemical composition of organism and the laws of chemical changes during the life process. The content of the course includes the following: ①structure and function of some biomacromolecules as protein, nucleic acid, carbohydrate, lipid and enzyme. ②metabolism of carbohydrate, lipid, amino acid and nucleotide in vivo.③ molecular basis and regulation laws in genetic information transferring.④cell signal transduction and extracellular matrix needful for various physiological activities.⑤ special biochemistry of some tissues and organs as blood and liver. This subject mainly adopts principle and methods of chemistry, and also melts the theory and skills of biophysics, physiology, cell biology, genetics and immunology. So, it has extensive interrelation and intersection with numerous subjects. Biochemistry and molecular biology is one of the important basic subjects for medicine.

Chapter 1 Introduction

[Content of course]

I.

II.

i.

ii.

iii.

III.

[Request]

I.

II.

III.

The concept of biochemistry. The development history of biochemistry Main contents of biochemistry course The chemical constitutes, molecule structure and function of organism The material metabolism and its regulation The reserve, deliver and expression of genetic information Biochemistry and medical science Grasp the concept of biochemistry Acquaint with the main contents of biochemistry Know the relation between the biochemistry and medical science

Chapter 2

Structure and function of protein

[Content of course]

I.

II. Importance of protein in life process Molecule constitute of protein

i. Chemical element of the protein

ii. Basic structure unit of protein—amino acids

iii. Peptide

iv. Protein classification

III. Molecular structure of protein

i. Primary structure

ii. Secondary structure

iii. Tertiary structure

iv. Quaternary structure

IV. Relationship between the structure and function of protein

i. Relationship of primary structure and function of protein

ii. Relationship of spatial structure and function of protein

V. Physics and chemistry property, separation and purification of protein

i. Physics and chemistry property

ii. Separation and purification

iii. Amino acids sequence analysis of polypeptide chain

iv. Spatial structure measurement of protein

[Request]

I. Know the characteristics of chemical element of protein, grasp the basic structure

of amino acids and acquaint with the classification and the physics and chemistry property of amino acids.

II. Grasp the basic concept of the peptide bond and peptide chain and the primary

structure characteristics of insulin. Know a few important bioactive peptide.

III.

IV. Know the principles of protein classification. Grasp the familiar type of secondary structure: alpha-helix, beta-pleated sheet,

beta-turn and random coil. Acquaint with the concept of the peptide unit, motif, and chaperon.

V. Grasp the concept of tertiary structure and domain. Analyze the tertiary structure

characteristics of ribonuclease.

VI. Grasp the concept of quaternary structure and analyze the quaternary structure

characteristics of hemoglobin ( Hb).

VII. Grasp the relationship between primary structure and function of

protein .Acquaint with the concept of "molecular disease".

VIII. Grasp the relationship between spatial structure and function of protein and

acquaint with relationship between the conformation variety and the function of binding to oxygen of hemoglobin.

IX. Grasp the ampholyte property, high molecular weight and denaturation of protein.

Acquaint with precipitation and of protein ultraviolet absorption.

X. Grasp the electrophoresis and gel filtration of protein. Know the salt precipitation,

isoelectric point, ultrafiltration and ultracentrifugation.

XI.

XII.

Know the principles of primary structure measurement of protein. Know the principle and meaning of the spatial structure estimation of protein.

Chapter 3

Structure and function of nucleic acid

[Content of course]

I. Chemical constitute of nucleic acid

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ii.

iii.

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III. Base component of nucleotide Pentose and nucleoside Structure and naming of nucleotide Primary structure of nucleic acid Spatial structure and function of deoxyribonucleic acid(DNA)

i.

ii.

iii. Secondary structure of DNA——double helix structure model Superhelix of DNA Function of DNA

IV. Spatial structure and function of ribonucleic acid(RNA)

i.

ii.

iii. Messenger ribonucleic acid (mRNA) Transfer ribonucleic acid (tRNA) Ribosomal ribonucleic acid (rRNA)

iv.

v.

V. Other small ribonucleic acid and RNomics Ribozyme Physics and chemistry property of nucleic acid and its application

i.

ii.

iii. General physics and chemistry property DNA denaturation DNA renaturation (annealing) and hybridization

VI.

Nuclease

[Request]

I. Grasp the structure, symbol and property of familiar nucleotides. Grasp the

molecular constitute of DNA and RNA. Grasp the connection pattern and the bond directivity of nucleotide. Grasp the primary structure of nucleic acid and its representational method.

II. Grasp the secondary structure of DNA and tRNA——double helix structure

model and cloverleaf pattern.

III. Grasp the denaturation, renaturation, unwinding curve and melting temperature of

DNA.

IV.

Know the following concepts: annealing, hybridization, ribozyme and nucleas …… 此处隐藏：19900字，全部文档内容请下载后查看。喜欢就下载吧 ……