PlantMolBiol(2010)72:407–421DOI10.1007/s11103-009-9579-6

Genome-widetranscriptomeanalysisoftwomaizeinbredlinesunderdroughtstress

JunZheng JunjieFu MingyueGou JunlingHuai YunjunLiu MinJian QuanshengHuang XiyingGuoZhigangDong HongzhiWang GuoyingWang

Received:2July2009/Accepted:16November2009/Publishedonline:2December2009ÓSpringerScience+BusinessMediaB.V.2009

AbstractDroughtstressgreatlyaffectsplantgrowthandcropyield.Tounderstandthetranscriptomedynamicsduringdroughtstressinmaizeseedlings,genome-widegeneexpressionpro lingwascomparedbetweenthedrought-tolerantlineHan21anddrought-sensitivelineYe478usingAffymetrixMaizeGenomeArraycontaining17,555probesets.Theresultsshowedthatinresponsetodrought,theHan21linehadfewerprobesetswithsignif-icantexpressionchangethantheYe478lineandbothlineshadacommonsetof*2,600regulatedprobesetsunder

TheauthorsJunZheng,JunjieFu,andMingyueGouarecontributedequallytothispaper.

droughtstress.Thepotentialcomponentsoftheabscisicacidsignalingpathwayweresigni cantlyidenti edfromthecommonprobesets.Atotalof827probesetswithsigni cantlydifferentialexpressionbetweenthetwolinesunderdroughtstresswereidenti ed.Thedifferentialexpressionlevelsofcellwall-relatedandtransportergenesmaycontributetothedifferenttolerancesofthetwolines.Additionally,wefoundthat,comparedtothesensitivelineYe478,thetranscriptionallevelsofdrought-responsiveprobesetsinthetolerantlineHan21recoveredmorequicklyafterre-watering,andmoreprobesetsinthetol-erantlineHan21wereexclusivelyup-regulatedatthere-wateringstage.Ourstudyprovidesaglobalgeneexpres-siondynamicsoftwomaizeinbredlinesduringdroughtstressandre-wateringandwillbevaluableforfurtherstudyofthemolecularmechanismsofdroughttoleranceinmaize.

KeywordsMaizeÁDroughtstressÁExpressionpro lingÁAbscisicacid

ElectronicsupplementarymaterialTheonlineversionofthisarticle(doi:10.1007/s11103-009-9579-6)containssupplementarymaterial,whichisavailabletoauthorizedusers.

J.ZhengÁY.LiuÁG.Wang(&)

InstituteofCropSciencesandNationalCenterforPlantGeneResearch,ChineseAcademyofAgriculturalSciences,SouthernStreetofZhongguancun12,100081Beijing,Chinae-mail:gywang@http://

J.FuÁM.GouÁJ.HuaiÁM.JianÁX.GuoÁZ.DongÁG.Wang

StateKeyLaboratoryofAgrobiotechnology,ChinaAgriculturalUniversity,100193Beijing,China

Q.Huang

InstituteofNuclearandBiologicalTechnology,XinjiangAcademyofAgriculturalScience,830000Urumqi,ChinaH.Wang

BeijingAgro-BiotechnologyResearchCenter,BeijingAcademyofAgricultureandForestryScience,100097Beijing,China

Introduction

Droughtstressisoneofthemajorenvironmentalstressesthatgreatlyaffectplantgrowthandcropproduction.Inordertosurvive,plantshaveadaptedtoexecuteanumberofmolecular,cellular,physiologicalandmetabolicresponsesduringdroughtstress(Bray1993;Bohnertetal.1995).Studieshaveshownthatvariousgeneswereinducedandinvolvedinthedroughtstressresponseinvariousplants(IngramandBartels1996;ShinozakiandYamagu-chi-Shinozaki1997;XiongandZhu2002).Theproductsoftheseinduciblegenesplayrolesnotonlyinstresstoler-ance,butalsointheregulationofgeneexpressionand

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408signaltransductioninstressresponses(Yamaguchi-ShinozakiandShinozaki2006).Theexistenceofavarietyofdroughtstress-induciblegenessuggeststhatthenatureoftheresponsetodroughtstressiscomplex.Recently,theArabidopsisgeneHDG11,whichencodesaHD-START-typetranscriptionfactor,wasreportedtodramaticallyimprovedroughttoleranceinArabidopsisandtobaccobyenhancingrootgrowthandreducingstomataldensity(Yuetal.2008).TheArabidopsisNF-YB(forBsubunitofnuclearfactorY)familytranscriptionfactorwasreportedtoconferdroughttoleranceinArabidopsisandmaize(Zeamays),suggestingapreviouslyunknownmolecularmech-anismofdroughttolerance(Nelsonetal.2007).Inaddi-tion,ithasbeenreportedthatoverexpressionofariceNAC-typetranscriptionfactorcouldimprovedroughttol-eranceinrice(Huetal.2006).Althoughsomemolecularelementshavebeenidenti ed,thegenenetworkofthedroughtstressresponseisstillnotfullyunderstood(Zhu2002).Thechallengeinthenearfutureremainstheidenti cationofmoremolecularelementsinresponsetodroughtstressandcharacterizationofusefulgenestoimprovedroughttoleranceinplants.

TranscriptomeanalysisusingcDNAmicroarraysorGeneChipshasbeenshowntobeapowerfultoolforthediscoveryofmorestress-induciblegenesinvolvedinstressresponseandtolerance(FowlerandThomashow2002;Ozturetal.2002;Shinozakietal.2003;Sekietal.2004).AcDNAmicroarraycontaining7,000full-lengthcDNAswasappliedtoidentifydrought-,cold-,salt-,andabscisicacid(ABA)-induciblegenesinArabidopsis(Sekietal.2002).AffymetrixGeneChipsthatcontainabout24,000geneswereusedtoidentifycold-responsivegenesinArabidopsis(Leeetal.2005).Previously,weusedcDNAmacroarraystomonitorexpressionof190maizecDNAsinresponsetoosmoticstress(Zhengetal.2004).AcDNAmicroarraycontainingabout2,500cDNAsfrommaizewasusedtomonitorgeneexpressionindevelopingmaizeendospermandplacenta/pediceltissuesduringwaterde citandre-watering(YuandSetter2003).Recently,themaizeexpressionpro lesduringresponsestoseveralabioticstresseswereanalyzedusinglongoligonucleotidearrays,whichcontainedabout58,000elements(Fernandesetal.2008).

Droughtstresscanaffectmaizeproductionatalmostallgrowthstages,includingtheseedlingstage,pre- oweringand oweringperiod,andgrain- llingstage.Inparticular,droughtstresscanaffectplantgrowthduringtheseedlingstage.Therehavebeenseveralreportsofdroughttoleranceanalysesbetweendifferentinbredlinesattheseedlingstage(Liuetal.2004;Wuetal.2007),whichdemonstratedthediversityofdroughttoleranceamongdifferentinbredlines.Inourlab,afterscreeningdozensofmaizeinbredlinesunderdroughttreatmentattheseedlingstage,wedeterminedthatinbredlineHan21hadahighseedling

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PlantMolBiol(2010)72:407–421

survivalrateafterre-watering,whereasinbredlineYe478seldomsurvivedafterre-watering.AlthoughbothHan21andYe478belongtoeliteinbredlines,thetwolineshavebeenuseddifferentlyinmaizehybridbreeding.InbredlineHan21,selectedfromtheShan …… 此处隐藏：56017字，全部文档内容请下载后查看。喜欢就下载吧 ……