T7体外转录试剂盒 加帽mMESSAGEmMACHINE T7 kit (Ambion(7)

Life Ambion T7启动子转录试剂盒说明书

mMESSAGE mMACHINE® KitIntroduction

(optional) Labeled nucleotide(s): [α-32P] UTP or [α-32P]CTP can be added to the

reaction as a tracer to facilitate quantitation of the RNA synthesized. Any specific activity is acceptable.

(optional) For purification of the synthesized RNA:

–Buffer- or water-saturated phenol/chloroform

–Isopropanol

–Spin Columns

mMESSAGE mMACHINE® Kit User Guide7