Life Ambion T7启动子转录试剂盒说明书

mMESSAGE mMACHINE® Kit

mMESSAGE mMACHINE® Kit Procedure

Procedure overviewFigure 2Transcription reaction assembly and incubationPreparation of template DNACapped transcription reaction assembly

1. “Thaw the frozen reagents” on page 11

2. “Assemble transcription reaction at room temp” on page 11

3. “Mix thoroughly” on page 11

4. “Incubate at 37°C, 1hr” on page 11

5. “(optional) Add 1µL TURBO DNase, mix well and incubate 15min at 37°C” on

page 12

Recovery of the RNA

1. “MEGAclear Kit” on page 12

2. “Lithium chloride precipitation” on page 12

3. “Spin column chromatography” on page 12

4. “Phenol:chloroform extraction and isopropanol precipitation” on page 13

10mMESSAGE mMACHINE® Kit User Guide