The Cauliflower mosaic virus (CaMV) open reading frame VI product (P6) is essential for the viral infection cycle. It controls translation reinitiation of the viral polycistronic RNAs and forms cytoplasmic inclusion bodies (viroplasms) where virus replicat

Figure8.TheLeu-EnrichedSequenceofDomainAIsResponsiblefortheNuclearExportofP6.

(A)SchematicrepresentationofEGFP:Aandmutatedversions.EGFP,domainA,andmotifI1ofP6arerepresentedasinFigure5.TheemptyspaceandtheredstarindicatethedeletionandthepointmutatedmotifI1,respectively.

(B)SubcellularlocalizationinBY-2cellsofEGFP:AI1andEGFP:Am(images1to4).The uorescentimages(1and3)observedbyCLSMaresuperposedonthecorrespondingdifferentialinterferencecontrastimages(2and4).

(C)EffectofleptomycinBonthesubcellularlocalizationofEGFP:Afusionproteinsandofwild-typeandpointmutatedformsofEGFP:P6.FluorescentcellswereselectedunderanHBOlamp,collectedinBY-2cellculturemediumcontaining100nMleptomycinB(imagestotheright)ornoleptomycin(imagestotheleft),andincubated6hat248Cwithgentleshaking.Thepoint-mutatedaminoacidswithintheI1sequenceareindicated.Bars¼10mm.