原版拟南芥规范种植手册

HANDLING ARABIDOPSIS PLANTS AND SEEDS

Methods used by the Arabidopsis Biological Resource Center

GROWTH OF PLANTS Planting on soil

Growth conditions

Control of pests

Plant isolation and harvesting

SEED HANDLING AND PRESERVATION

The methods used by the ABRC for handling plants and seeds are outlined below. These procedures are designed to generate healthy plants that give maximum set of pure seeds and to preserve these in the safest and most convenient manner. Many other approaches may be equally as good, especially in specific experimental situations.

GROWTH OF PLANTS

Arabidopsis can be grown in a variety of environmental settings including growth rooms, window ledges, outdoors, growth chambers and greenhouses.

Peat moss-based mixes, commercial greenhouse mixes, relatively inert media watered with nutrient solutions, and defined agar media can all be employed as plant substrates.

Our focus will be on growth of plants on agar and soil in growth chambers and

greenhouses. The plant and seed management methods are discussed in the chronological order in which they would normally be utilized.

It is necessary to use sterile conditions to grow Arabidopsis for specific experiments such as selection of transformed plants, drug resistant plants, early root and shoot phenotypes, lethal mutants, etc. Otherwise, contaminants can essentially take over plant cultures. Various shapes and sizes of containers such as petri dishes, 'Magenta' boxes, or culture tubes can be used, depending on the required length of the growing time (2-3 weeks or to maturation) and characterization of phenotypes (shoot or roots). We will emphasize the