T载体

pUC-T Easy Vector Systems

INSTRUCTIONS FOR USE OF PRODUCTS T1101

and 900μl to the uncut DNA control tube. Incubate for 1.5 hours at 37°C with shaking (~150rpm) .

7. Plate 100μl of each transformation culture onto duplicate LB/ampicillin/IPTG/X-Gal plates. For the uncut DNA control, a 1:10 dilution with SOC is recommended.

8. Incubate plates overnight at 37°C. Select white colonies.

5

CAGCACTGACCCTTTTGGGACCGC

BcaBestTM Sequencing Primer M13-47